• Mostafa, M. E.; Grinias, J. P.; Edwards, J. L., Supercritical Fluid Chromatography-Nanospray Ionization Mass Spectrometry . Journal of Chromatography A 2024, 465377. https://doi.org/10.1016/j.chroma.2024.465377

  • Agongo, J.; Grady, S.F.; Cho, K.; Patti, G.J.; Bythell, B.J.; Arnatt, C.J.; Edwards, J.L. Discovery and Identification of Three Homocysteine Metabolites by Chemical Derivatization and MS Fragmentation. Analytical Chemistry 2024. https://doi.org/10.1021/acs.analchem.4c01706

  • Mostafa, M. E.; Agongo, J.; Grady, S. F.; Pyles, K.; McCommis, K.; Arnatt, C. K.; Ford D. A.; Edwards, J. L., Double Cyclization Tandem Mass for Identification and Quantification of Phosphatidylcholines Using Isobaric Six-Plex Capillary nLC-MS/MS. J Am Soc Mass Spectrom 2024. https://doi.org/10.1021/jasms.3c00447

  • Armbruster, M.; Grady, S.; Cho, K.; Patti, G.; Bythell, B.; Arnatt, C.; Edwards, J. L., High Throughput Metabolomics using 96-plex Isotope Tagging. Analytical Chemistry 2024 https://doi.org/10.1021/acs.analchem.4c02279.

  • Saw, Y. L.; Boughton, J. R.; Wroniuk, F. L.; Mostafa, M. E.; Pellegrinelli, P. J.; Calvez, S. A.; Kaplitz, A. S.; Perez, L. J.; Edwards, J. L.; Grinias, J. P., Use of N-(4-aminophenyl)piperidine Derivatization to Improve Organic Acid Detection with Supercritical Fluid Chromatography-Mass Spectrometry. Journal of Separation Science 2023; DOI: 10.1002/jssc.202300343.

  • Armbruster, M.; Grady, S. F.; Caldwell, R.N.; Arnatt, C. K.; Edwards, J. L., Dual Fragmentation Isobaric Tags for metabolomics. J Am Soc Mass Spectrom 2023. DOI:10.1021/jasms.3c00171

  • Mostafa, M. E.; Hayes, M. M.; Grinias, J. P.; Bythell, B. J.; Edwards, J. L., Supercritical Fluid Nanospray Mass Spectrometry II. Effects on Ionization. J Am Soc Mass Spectrom 2023. DOI:10.1021/jasms.2c00372

  • Armbruster, M.; Mostafa, M. E.; Caldwell, R.N.; Grady, S. F.; Arnatt, C. K.; Edwards, J. L., Isobaric 6-plex and tosyl dual tagging for the determination of positional isomers and quantitation of monounsaturated fatty acids using rapid UHPLC-MS/MS. Analyst 2022, DOI: 10.1039/D2AN01699K. Analyst HOT Article 2023

  • Mostafa, M. E.; Grinias, J. P.; Edwards, J. L., Supercritical Fluid Nanospray Mass Spectrometry. J Am Soc Mass Spectrom 2022. 33 (10), 1825-1832, DOI: 10.1021/jasms.2c00134

  • Agongo, J.; Armbruster, M.; Arnatt, C.; Edwards, J., Analysis of Endogenous Metabolites using Multifunctional Derivatization and Capillary RPLC-MS. Analytical Methods 2022.

  • Mostafa, M. E.; Grinias, J. P.; Edwards, J. L., Evaluation of Nanospray Capillary LC-MS Performance for Metabolomic Analysis in Complex Biological Matrices. Journal of Chromatography A 2022, 462952.

  • Currens, E. R.; Armbruster, M. R.; Castiaux, A. D.; Edwards, J. L.; Martin, R. S., Evaluation and optimization of PolyJet 3D-printed materials for cell culture studies. Analytical and Bioanalytical Chemistry 2022.

  • Armbruster, M. R.; Grady, S. F.; Arnatt, C. K.; Edwards, J. L., Isobaric 4-Plex Tagging for Absolute Quantitation of Biological Acids in Diabetic Urine Using Capillary LC–MS/MS. ACS Measurement Science Au 2022.

  • Armbruster, M.; Grady, S.; Agongo, J.; Arnatt, C. K.; Edwards, J. L., Neutron encoded derivatization of endothelial cell lysates for quantitation of aldehyde metabolites using nESI-LC-HRMS. Analytica Chimica Acta 2022, 1190, 339260.

  • Coulton, J.B.; Edwards, J.L. “Capillary flow-based sample preparation system for metabolomic analysis of mammalian cells in suspension” Analytical and Bioanalytical Chemistry, 2021.

  • Kaplitz, A.S.; Mostafa, M.E.; Calvez, S.A.; Edwards, J.L.; Grinias, J.P. “Two-Dimensional Separation Techniques Using Supercritical Fluid Chromatography” Journal of Separation Science. 2021; 44:426-437. Top Cited Article 2021, 2022.

  • Sanders, K.L.; Edwards, J.L. “Nano-Liquid Chromatography-Mass Spectrometry and Recent Applications in Omics Investigations” Analytical Methods, 2020,12, 4404-4417.

  • Goll, J.B.; Li, S.; Edwards, J.L.; Bosinger, S.E.; Jensen, T.L.; Wang, Y.; Hooper, W.F.; Gelber, C.E.; Sanders, K.L.; Anderson, E.J.; Rouphael, N.; Natrajan, M.S.; Johnson, R.A.; Sanz, P.; Hoft, D.; Mulligan, M.J. “Transcriptomic and Metabolic Responses to a Live-Attenuated Francisella tularensis Vaccine.” Vaccines 8(3),412, pp. 1-23.

  • Guan, S.; Armbruster, M.R.; Huang, T.; Edwards, J.L.; Bythell, B.J. “Isomeric Differentiation and Acidic Metabolite Identification by Piperidine-based Tagging, LC-MS/MS, and Understanding of the Dissociation Chemistries.” Analytical Chemistry 2020, 92, 13, 9305–9311.

  • Edwards, T.H.; Koenig, A.; Thomas, L.; Sadik, A.; Edwards, J.L.;, “Purification of Canine Albumin by Heat Denaturation in a Plasma Bag ” Journal of Veterinary Emergency and Critical Care 2020, 30, 264-271.

  • Filla, L.A.; Sanders, K.L.; Coulton, J. B.; Filla, R.T.; Edwards, J.L. “Determination of online quenching efficiency for an automated cellular microfluidic metabolomic platform using mass spectrometry based ATP degradation analysis” Analytical and Bioanalytical Chemistry 2019, 411, 6399-6407.

  • Huang, T.; Bythell, B.J.; Edwards, J.L. “Fragmentation of Multi-charged Derivatized Lysine Using Nanospray CID Tandem Mass Spectrometry” J Am Soc Mass Spectrom. 2019, 30 (7) 1158-1162.

  • Huang, T.; Armbruster, M.R.; Coulton, J.B.; Edwards, J.L. “Chemical Tagging in Mass Spectrometry for Systems Biology.” Analytical Chemistry 2019, 91 (1) 109-125.

  • Li, L.; Liu, X.; Sanders, K. L.; Edwards, J. L.; Ye, J.; Si, F.; Gao, A.; Huang, L.; Hsueh, E. C.; Ford, D. A.; Hoft, D. F.; Peng, G., “TLR8-Mediated Metabolic Control of Human Treg Function: A Mechanistic Target for Cancer Immunotherapy.” Cell Metabolism 2019, 29 (1) 103-123.

  • Huang, T.; Armbruster, M.R.; Hui, D.S.; Lee, R.; Edwards, J.L. "Metabolomic Analysis of Mammalian Cells and Human Tissue through One-Pot Two Stage Derivatizations using Sheathless CE-ESI-MS” Journal of Chromatography A, 2018, 1567, 219-225.

  • Huang, T.; Hui, D.; Lewis, M.; Toro M.D.; Edwards, J.L. “Multi-Functional Derivatization of Amine, Hydroxyl, and Carboxylate Groups for Metabolomic Investigations of Human Tissue by Electrospray Ionization Mass Spectrometry” Analyst, 2018, 143, 3408-3414.

  • Zhao, X.F.; Hui, D.; Edwards, J.L. “Ratiometric Quantitation of Thiol Metabolites using non-isotope Maleimide Tags” Analytica Chimica Acta, 2018, 1037, 274-280.

  • Filla, R.T.; Schrell, A.M.; Coulton, J.B.; Edwards, J.L.*, Roper, M.G.* "Frequency-Modulated Continuous Flow Analysis

    Electrospray Ionization Mass Spectrometry for Sample Multiplexing"  Analytical Chemistry, 2018, 90 (4), 2414-2419.

  • Filla, L.A.; Sanders, K.L.; Filla, R.T.; Edwards, J.L. “Automated Sample Preparation in a Microfluidic Culture Device for Cellular Metabolomics” Analyst 2016 141 (12) 3858-3865. Invited Emerging Investigators Issue.

  • Forzano, A.V.; Becirovic, V.; Martin, R.S.; Edwards, J.L. “Integrated Electrodes and Electrospray Emitter for Polymer Microfluidic Nanospray-MS Interface” Analytical Methods 2016, 8, 5152-5157.

  • Filla, L.A.; Edwards, J.L. “Metabolomics in Diabetic Complications” Molecular Biosystems 2016, 12, 1090-1105.

  • Esch, C.; Hui, D.S.; Lee, R.; Edwards, J.L. “Quantitation of Thiol Metabolites from Mammalian Cells using Fluorous Tagging and HILIC-MS” Analytical Methods 2015 7, 7164-7169. Invited for Young Investigator Issue.

  • Yuan, W. and Edwards J.L. “Extraction and Quantitation of Ketones and Aldehydes from Mammalian Cells using Fluorous Tagging and Capillary LC-MS.” Anal. Chem. 2015, 87, 7660-7666.

  • Filla, L; Yuan, W, Li, S.; Feldman E.L.; Edwards, J.L. “Global Metabolomic and Isobaric Tagging capLC-MS Approaches For Uncovering Pathway Dysfunction in Diabetic Mouse Aorta.” Journal of Proteome Research. 2014 13, 6121-6134.

  • Yuan, W.; Li, S; Edwards J.L. “Global Profiling of Carbonyl Metabolites with a Photo-cleavable Isobaric labeling Affinity Tag” Chem. Comm. 2013 49, 11080-11082.

  • Yang, S.; Yuan, W.; Yang, W.; Zhou, J.; Harlan, R.; Edwards, J; Li, S.; Zhang, H. “Glycan Analysis by Isobaric Aldehyde Reactive Tags and Mass Spectrometry” Anal. Chem. 2013, 85, 8188-8195.

  • Chemnitz, W.; Yuan, W.; Edwards, J.; Kelman, Z.; Marino, J. Hurwitz, J.; “Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakarensis DNA primase” J Biol Chem. 2012 287, 16220-16229.

  • Chen, Z.; Wang, Q.; Lin, L.; Tang, Q.; Edwards, J.L.; Li, S.; Liu, S. “Comparative Evaluation of Two Isobaric Labeling Tags, DiART and iTRAQ.” Anal. Chem. 2012, 84, 2908-2915.

  • Yuan, W.; Anderson, K.; Li, S; Edwards J.L. “Sub-Second Absolute Quantitation of Amine Metabolites for Discovery of Pathway Activation in Mammalian Cells.” Anal. Chem. 2012, 84, 2892-2899.

  • Yuan, W.; Anderson, K.; Li, S; Edwards J.L. “Quantitation of Amine Metabolites in Hyperglycemic Endothelial Cells using Capillary LC-MS/MS with Isobaric Tagging.” Journal of Proteome Research 2011, 10, 5242-5250.

  • Li, Z.; Pan, M.; Santangelo, T.; Chemnitz, W.; Yuan, W.; Edwards, JL.; Hurwitz, J.; Reeve, J.; Kelman, Z. “A novel DNA nuclease is stimulated by association with the GINS complex” Nucleic Acids Res. 2011, 39, 6114-6123.

  • Yuan, W.; Edwards, J.L. “Thiol Metabolomics of Endothelial Cells using Capillary Liquid Chromatography Mass Spectrometry with Isotope Coded Affinity Tags” J. Chrom. A. 2011, 1218, 2561-2568.

  • Vincent, A.M., Edwards, J.L.; McLean, L.L., Hong, Y; Cerri, F; Lopez, I., Quattrini, A; Feldman, E.L. “Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy” Acta Neuropath 2010, 120, 477-489.

  • Yuan, W.; Edwards, J.L. “Metabolomics in Capillary Separations” Bioanalysis. 2010, 2, 953-963.

  • Lentz,S.I.; Edwards, J.L.; Haines, K; Backus, C; McLean, L; Feldman, E.L. “Mitochondrial DNA (mtDNA) Biogenesis: Enhanced Visualization of BrdU and EdU Incorporation in Newly Synthesized mtDNA In Vitro” Journal of Histochemistry and Cytochemistry. 2010, 58, 207-218.

  • Edwards, J.L.; Quattrini, A; Lentz, S.I.; Figueroa-Romero, C; Cerri, F; Backus, C; Hong, Y; Feldman, E.L. “Diabetes Regulates Mitochondrial Biogenesis and Fission in Mouse Neurons” Diabetologia 2010, 53, 160-169.

  • Edwards, J.L.; Vincent, A.M.; Cheng, H.L.; Feldman, E.L. “Diabetic Neuropathy: Mechanisms to Management” Pharmacol Ther. 2008, 120, 1-34.

  • Vincent, A.M.; Edwards, J.L.; Sadidi, M.; Feldman, E.L. “The Antioxidant Response as a Drug Target in Diabetic Neuropathy” Curr Drug Targets 2008, 9, 94-100.

  • Edwards, J.L.; Edwards, R.L.; Reid, K.A.; Kennedy, R.T. “Effect of Decreasing Column Inner Diameter and Use of Off-line Two-Dimensional Chromatography on Metabolite Detection in Complex Mixtures” J. Chrom. A. 2007, 1172, 127-134.

  • Little, A.A.; Edwards, J.L.; Feldman, E.L. “Diabetic Neuropathies” Practical Neurology 2007, 7, 82-92.

  • Leinninger, G.M.; Edwards, J.L.; Lipshaw, M.J.; Feldman, E.L. “Mitochondria as New Therapeutic Targets in Diabetic Neuropathy” Nature Clinical Practice Review 2006, 11, 620-628.

  • Edwards, J.L.; Chisolm, C.; Shackman, J.G.; Kennedy, R.T. “Negative Mode Sheathless Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry for Metabolite Analysis of Prokaryotes” J. Chrom. A. 2006, 1106, 80-88.

  • Edwards, J.L.; Kennedy, R.T. “Metabolomic Analysis of Eukaryotic Tissue and Prokaryotes using Negative Mode MALDI Time of Flight Mass Spectrometry” Anal. Chem. 2005, 77, 2201-2209.

  • Shim, J.Y.; Welsh, W.J.; Cartier, E.; Edwards, J.L.; Howlett, A.C. “Molecular Interaction of the Antagonist N-(Piperidin- 1-yl)- 5-(4- chlorophenyl)-1(2,4- dichlorophenyl)-4- methyl-1H- pyrazole-3-carboxamide with the CB 1 Cannabinoid Receptor” J. Med. Chem. 2002, 45, 1447-1459.

  • Edwards, J.; Sprung, R.; Sprague, R.; Spence, D. “Chemiluminescence Detection of ATP Release from Red Blood Cells upon Passage through Microbore Tubing” Analyst. 2001 126: 1257-1261.

  • Edwards, J.; Patel, A.; Spence, D. “Performance Enhancement in Flow Reversal Flow Injection using On-Capillary Detection” Anal. Chim. Acta. 2000, 417: 185-190.

  • Edwards, J.L.; Bauman, R.; Spence, D.M. “Air Segmented Continuous Flow Analysis in Microbore Tubing” Anal. Chim. Acta. 1999, 401: 209-214.

Department of Chemistry and Biochemistry

Saint Louis University Phone: (314)977-3624

3501 Laclede Ave Room 228; E-mail: jim.edwards@slu.edu

St. Louis, MO 63103